Web520 nm) Fluorescein isothiocyanate (FITC) is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical … WebOur fluorochrome chart includes Aligned emission and excitation fluorescence spectra for 30 of the most commonly used fluorochromes, including tandem dyes Easy visualization of some of the most popular …

How does fluorochromes work? - Kromnigon

WebFLUOROCHROME: EXCITATION(nm) EMISSION (nm) 3-Hydroxypyrene 5,8,10-Tri Sulfonic acid: 403: 513: 5-Hydroxy Tryptamine: 380-415: 520-530: 5-Hydroxy Tryptamine … WebFigure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 690 dye. Click image to enlarge Figure 2. Spectral signature of NovaFluor Yellow 690 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 690 dye were used for analysis. the pioneer woman ice cream maker

Fluorophores

Webnm, with an emission peak wavelength of 509 nm. The wild type like variants have their primary excitation peak centered on 395 nm, with an emission peak at 509 nm while the blue emitting mutants generally have an excitation peak at around 380 nm and an emission peak near 460 nm (Figure 1). Table 1. Excitation and emission data of GFP variants GFP WebSep 11, 2024 · A typical fluorochrome absorption-emission spectral diagram is illustrated in Figure 2. Note that the curves of fluorescence intensity for absorption (usually similar to the excitation curve for pure … WebEmission filter (nm) Channel Recommended dyes Viability dyes (compatible with fixation) Viability dyes (non-fixed cells) DNA content/cell cycle dyes (live cells) DNA content/cell cycle dyes (fixed cells) Apoptosis dyes Cell proliferation dyes ROS detection dyes Phagocytosis dyes Fluorescent proteins Other dyes Violet (405 nm) 440/50 VL1 Alexa ... the pioneer woman is also known as