Hu-binding site

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August undefined, 2024

Web1 Dec 2000 · HU binds DNA junctions and DNA containing a nick much more avidly than double-stranded (ds-) DNA. Cells lacking HU are extremely sensitive to γ irradiation and … WebBinding Site provides specialist diagnostic products to clinicians and laboratory professionals worldwide. Our people are dedicated to improving patient lives, …

A second high affinity HU binding site in the phage Mu

Web29 Apr 2014 · Site-directed mutagenesis of the putative C/EBPα binding sites decreased FTO promoter activity. Overexpression and RNAi studies also indicated that C/EBP α was required for the expression of FTO. Chromatin immunoprecipitation (ChIP) experiment was carried out and the result shows direct binding of C/EBP α to the putative binding … Web22 Mar 2011 · HU acts to compact DNA at physiological salt concentrations in vitro by introducing bends ranging from 53° to more than 140° in the DNA as assayed by X-ray crystallography, gel electrophoresis, DNA cyclization, atomic force microscopy and fluorescence resonance energy transfer (FRET). pro cycle shorts

Repressor induced site‐specific binding of HU for transcriptional ...

Web3 Jun 1994 · The bacteriophage Mu in vitro transposition reaction proceeds through several higher order nucleoprotein intermediates (transpososomes). One of the requirements for complex assembly is the Escherichia coli sequence-independent DNA-binding protein, HU. This protein has an affinity for Mu transpososomes, which is at least 100 times greater … Web22 Mar 2011 · If the binding site for HU is estimated to be 21 bp per dimer, based on co-crystal structures , the maximum number of bound HU on λ DNA would be ~2300 dimers. … Web1 Mar 2008 · HU binding to ssDNA, dsDNA and nick in low salt conditions. HU protein, at the concentration indicated in nM on the bottom, was mixed with end-labeled DNA, 40-bp long dsDNA, the same DNA with a ... pro cycle speedometer

A second high affinity HU binding site in the phage Mu …

HU: promoting or counteracting DNA compaction?

Web11 Dec 2024 · In 2012, Lu et al. [ 15] used the “fragment transformation” method to predict metal ion (Ca 2+, Mg 2+, Cu 2+, Fe 3+, Mn 2+, Zn 2+) ligand binding sites, and the prediction results were obtained with a total accuracy of 94.6% and a true positive of rate 60.5%. In 2016, Hu et al. [ 16] identified four metal ions in the BioLip database by both ... WebHU binding site and present evidence that protein-protein in- teractions do not mediate this high affinity binding. Further- more, we report that the mammalian HMG-1 protein … procycles stuart flWeb2 Sep 2013 · The HU protein bends DNA at low protein concentrations, promoting compaction, and stiffens DNA at high concentrations (supplementary figure S1). (6-8) The affinity of HU for short regular B-DNA substrates is in the micromolar range, whereas affinities in the nanomolar range have been reported for binding to pre-distorted DNA … reinforced carrypod

"Web19 Mar 2024 · The PWSM has been successfully used in the prediction of transcription factor binding sites and ligand binding sites (Kel et al., 2003; Hu et al., 2016a). Thus, the position weight scoring matrix (PWSM) was used to extract the position conservation information of the basic feature parameters, and the scoring matrix based on amino acid … " - Hu-binding site

Hu-binding site

Web19 Jun 2008 · The histone-like HU (heat unstable) protein plays a key role in the organization and regulation of the Escherichia coli genome. The nonspecific nature of HU binding to DNA complicates analysis of the mechanism by which the protein contributes to the looping of DNA. Web26 Feb 2024 · Starting from a query sequence and predicted I-TASSER models, ATPbind can achieve an average accuracy of 72%, covering 62% of all ATP binding sites while achieving a Matthews correlation coefficient value that is significantly higher than that of other state-of-the-art predictors. Publication types Research Support, N.I.H., Extramural

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WebHu Proteins Block Binding of CstF64 to Poly(A) Sites and Interact with Two Polyadenylation Factors—To probe the mechanism whereby Hu proteins inhibit … Web4 Jul 2013 · The dimer to base pair ratio at 50 nM HU is 1:8. This ratio can be considered as the onset of the high concentration regime. It has been reported that HU binds non-specifically to a 9 bp binding site at high concentrations of HU ( 20, 22, 31). In all aforementioned cases, 50 nM HU (1 dimer: 8 bp), a temporary stiffening is observed, …

Web22 Mar 2011 · HU acts to compact DNA at physiological salt concentrations in vitro by introducing bends ranging from 53° to more than 140° in the DNA as assayed by X-ray … WebPapain-like protease (PLpro) is a promising therapeutic target against SARS-CoV-2, but its restricted S1/S2 subsites pose an obstacle in developing active site-directed inhibitors. We have recently identified C270 as a novel covalent allosteric site for SARS-CoV-2 PLpro inhibitors. Here we present a theoreti

Web1 Aug 2011 · The Hu proteins (HuA/R, HuB, HuC, and HuD) are a family of mammalian RNA-binding proteins. Of the four Hu family members, HuA/R is widely expressed in … Web1 Mar 2008 · HU binds single-stranded DNA (ssDNA) in a binding mode that is reminiscent but different from earlier reported specific HU interactions with double-helical DNA …

Web20 Dec 2007 · HU binding induces FRET between labeled ssDNA ends. Very long apparent DNA-binding sites and abnormalities in gel mobility of protein–DNA complexes often …

reinforced cardboard boxWebCompared to the Mn1-, Mn2- and Mg-doped TiO2 nanocoatings, the Mn3-TiO2 nanocoating significantly upregulated the expression of integrin α5β1 probably through increasing ligand-binding affinity of the integrin rather than integrin binding sites in Fn. reinforced carbon fiberWeb15 Jun 1997 · Site-specific binding of HU is promoted by GalR binding and supercoiled DNA Affinity cleavage mapping by chemically converting HU into a nuclease gives information about the position of the reactive groups in HU in the DNA–protein complex ( Lavoie and Chaconas, 1993; Lavoie et al ., 1996 ). procyclical behavior of velocityWeb16 Sep 2024 · To evaluate the possible influence of the labelling site on the binding and diffusion behavior of HU on DNA, we generated a simple model of a HU–DNA complex, based on the IHF-DNA complex ... reinforced cell frameWebThe binding sites in c-Fos, interleukin-3, c-Myc, Tau, IVA 2 , and GAP-43 mRNAs are shown. ... Neuronal ELAV/Hu (nELAV) are RNA-binding proteins that mainly regulate … reinforced cement boardWebbound to its recognition site. Binding of HU is sequence un-speci¢c but, as is the case with IHF [7], binding to DNA probably involves its two arms being inserted into the minor groove. H-NS is similar in size to HU (15.6 kDa) and also binds DNA without sequence speci¢city. Unlike HU, this protein reinforced cardboard sheetsWeb20 Dec 2007 · The nucleoid-associated protein HU plays an important role in bacterial nucleoid organization and is involved in numerous processes including transposition, … procyclical budget