Klf2 shrna

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August undefined, 2024

WebStart gene knockdown with ready-to-use Klf2 mouse shRNA lentivirus , then verify knockdown efficiency with unique designed RT-PCR primers. Lentiviral Klf2 mouse shRNA … WebApr 15, 2024 · Western blot was performed to analyze the effects of KLF2 shRNA on knockdown of KLF2 expression and p15 and p21 expression in cells. We found that KLF2 …

Discovery of Klf2 interactors in mouse embryonic stem cells by ...

WebFeb 10, 2008 · Normal undifferentiated ES colonies with positive alkaline phosphatase staining were maintained after knockdown using two shRNA constructs targeting different regions of Klf2 or Klf5, respectively. WebDiabetic mice injected with KLF2 shRNA-lentivirus or control virus were treated with 20 mg/kg phloretin. Glucose, lipid profile, aortic atheroma, and endothelial nitric oxide synthase (eNOS) expression were detected. Phloretin retained endothelial function by KLF2-eNOS activation under hyperglycemia. discontinuous insulation of an axon

KLF2 Gene - GeneCards KLF2 Protein KLF2 Antibody

WebSep 5, 2011 · We strengthened the link between EZH2 expression and KLF2 repression by establishing a U2OS cell line stably transfected with a short hairpin RNA (shRNA) against … WebHere, we demonstrate a novel mechanistic relationship between p66shc and the kruppel like factor-2 (KLF2) transcription factor and show that this relationship has biological … WebOct 31, 2024 · For KLF2 knockdown, a human KLF2 gene-specific silencing and control lentivirus vector short hairpin RNA (shRNA) was developed, and the corresponding lentivirus particles were produced. Then, the KLF2 and control knockdown lentiviruses were concentrated using a density gradient centrifuge and titrated to determine virus efficiency. fourche rst neon

Silencing of O-linked N-acetylglucosamine transferase ... - Nature

Silencing of Kruppel-like factor 2 by the histone ... - Nature

WebWestern blot was performed to analyze the effects of KLF2 shRNA on knockdown of KLF2 expression and p15 and p21 expression in cells. We found that KLF2 expression was … WebFAM83A and DDIT4 expression was knocked-downed in leukemia cells via lentivirus-shRNA. Western blotting, RT-qPCR, cell cycle analysis and apoptosis assays were used to determine the effect of lovastatin-induced growth suppression in leukemic cells in vitro. ... We further show that KLF2 induction by lovastatin is responsible for lower ... discontinuous growth modelingKlf2 expression in this location plays a key atheroprotective role, preventing local inflammation and the subsequent formation of atherosclerotic lesions. We therefore tested whether NMP administration could influence gene expression in the aortic arch. To this end, ApoE−/− mice were fed with HFD and treated … See more Previous studies have demonstrated that NMP can affect stem cell differentiation by promoting Bone Morphogenic Protein (BMP)-dependent responses44. In … See more We reasoned that NMP's status as an FDA-approved pharmaceutical excipient might make it an attractive solvent with intrinsic therapeutic activities for treating … See more Having shown that NMP exerts an anti-inflammatory effect in cultured cells and that it is also well tolerated and active in vivo, we tested NMP in an animal model of … See more Our in vivo data in an established murine model of atherosclerosis drove our study towards human cells. For these experiments, we utilized human THP‐155 … See more discontinuous innovation product examples

"WebSilencing of KLF2 with KLF2 shRNA abolished the effects of laquinimod in inhibiting the expression of endothelial adhesion molecules and attachment of U937 monocytes to … " - Klf2 shrna

Klf2 shrna

Original Article Methylation of kruppel-like factor 2 (KLF2) …

WebMethods: Genetic manipulations of KLF2 were performed in bEnd.3 cells and SCI model. Western blot, qRT-PCR, immunofluorescence staining and Lyso-Tracker Red staining, Evans blue dye extravasation, behavioral assessment via Basso mouse scale (BMS), electrophysiology and footprint analysis were performed. WebWestern blot was performed to analyze the effects of KLF2 shRNA on knockdown of KLF2 expression and p15 and p21 expression in cells. We found that KLF2 expression was significantly reduced in NSCLC cells and tissues via KLF2 methylation.

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WebMay 7, 2024 · These results confirm that this dual transduction protocol does not affect A20's ability to increase KLF2, KLF4, and eNOS expression levels. Transduction of HCAEC … WebHuman KLF2 (10365), Mouse Klf2 (16598), Rat Klf2 (306330), chicken... Summary: Kruppel-like factors (KLFs) are a family of broadly expressed zinc... Related Products: Antibodies …

WebSep 1, 2024 · For shRNA-mediated knockdown of Klf2 and Snd1, DNA fragments targeting Klf2 and Snd1 were subcloned into pLKO.1-TRC vector (Addgene). Lentiviral vectors were co-transfected into HEK293T cells together with pCMV-VSV-G (Addgene) and psPAX2 (Addgene) plasmids using Lipofectamine 3000 reagent (Invitrogen), and viruses were … WebNov 23, 2024 · Background: Circular RNAs (circRNAs), as a class of endogenous RNAs, are implicated in osteosarcoma (OS) progression. However, the functional properties of circDOCK1 in OS have been largely unexplored. The present study demonstrated the regulatory mechanism of circDOCK1 in OS. Methods: QRT-PCR and Western blots were …

WebApr 25, 2024 · Silencing KLF2 with KLF2-specific shRNA aggravates the damage to hyperoxia-caused cell viability. In addition, as illustrated in Figures 6(d)–6(g), KLF2 shRNA … WebMEF2A protein and mRNA are more abundant in p66shc-knockout MEFs, resulting in greater occupancy of the KLF2 promoter by MEF2A. In endothelial cells, the increase in KLF2 and thrombomodulin protein by shRNA-induced decrease in p66shc expression is partly abrogated by knockdown of MEF2A.

WebAug 27, 2015 · As expected, single knock down of either gene has no obvious effect on mESC self-renewal in 2i condition (data not shown). We then selected the shRNAs with the highest knockdown efficiency (Klf2 … fourche rst vibe 110-15WebSep 5, 2011 · Conversely, stable depletion of KLF2 by shRNA in the non-transformed MRC5 fibroblast cells increased cell viability as assessed by the MTT assay . We also wished to investigate the contribution of ... discontinuous lease in reversionWebrally delivered KLF2 shRNA inhibited KLF2 mRNA by 50% to 60% compared to an empty lentiviral vector (Figure 1D) or a nonsilencing siRNA control vector (supplemental Figure II). Although KLF2 knockdown under shear did not affect Nrf2 mRNA, it reduced expression levels of NQO1 (P 0.01) and showed a decreasing trend in HO-1 expression compared fourche rodeo labWebSep 8, 2015 · Klf2 and Tfcp2l1 Knock Down Impairs 2i-Mediated mESC Self-Renewal (A) qRT-PCR analysis of Klf2 expression in Klf2 shRNA knockdown 46C mESCs. Data represent mean ± SD of three biological replicates. ∗∗ p < 0.01 versus scramble shRNA. (B) Western blot analysis of KLF2 expression in Klf2 shRNA knockdown 46C mESCs. discontinuous in mathWebJul 28, 2024 · Overexpression of KLF2 by adenovirus KLF2 (Ad-KLF2) in HUVECs reversed the inhibitory effect of OSS on UCP2 mRNA expression , suggesting that KLF2 can increase UCP2 expression. However, … discontinuous leave shared parental leaveWebNov 22, 2024 · Endothelial–mesenchymal transition occurs during intimal hyperplasia and neointima formation via mechanisms that are incompletely understood. Endothelial MAPK7 signaling is a key mechanosensitive fac... discontinuous growthWebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. fourche rst zeta el